Javascript kuri ubu irahagarikwa muri mushakisha yawe.Iyo javascript ihagaritswe, ibikorwa bimwe byuru rubuga ntibikora.
Andika amakuru yawe yihariye nibiyobyabwenge byihariye, kandi tuzahuza amakuru utanga ningingo muri data base yacu kandi twohereze kopi ya PDF ukoresheje imeri mugihe gikwiye.
Igenzura urujya n'uruza rwa magnetiki fer oxyde nanoparticles kugirango igere kuri cytostatike
Umwanditsi Toropova Y, Korolev D, Istomina M, Shulmeyster G, Petukhov A, Mishanin V, Gorshkov A, Podyacheva E, Gareev K, Bagrov A, Demidov O
Yana Toropova, 1 Dmitry Korolev, 1 Maria Istomina, 1,2 Galina Shulmeyster, 1 Alexey Petukhov, 1,3 Vladimir Mishanin, 1 Andrey Gorshkov, 4 Ekaterina Podyacheva, 1 Kamil Gareev, 2 Alexei Bagrov, 5 Oleg Demidov6,71Al Ikigo cy’ubushakashatsi cya Minisiteri y’ubuzima y’Uburusiya, St. Petersburg, 197341, Uburusiya;2 Ishuri rikuru ry’amashanyarazi rya St. Petersburg “LETI”, St. Petersburg, 197376, Uburusiya;3 Ikigo cy’ubuvuzi bwihariye, Ikigo cy’ubushakashatsi cy’ubuvuzi cya Leta ya Almazov, Minisiteri y’ubuzima y’Uburusiya, St. Petersburg, 197341, Uburusiya;4FSBI “Ikigo cy’ubushakashatsi cy’ibicurane cyitiriwe AA Smorodintsev” Minisiteri y’ubuzima y’Uburusiya, St. Petersburg, Uburusiya;5 Ikigo cya Sechenov Institute of Evolutionary Physiology and Biochemistry, Academy of Science of Russian, St. Petersburg, Uburusiya;6 RAS Institute of Cytology, St. Petersburg, 194064, Uburusiya;7INSERM U1231, Ishami ry’Ubuvuzi na Farumasi, Bourgogne-Franche Comté University ya Dijon, mu Bufaransa Itumanaho: Yana ToropovaAlmazov Ikigo cy’ubushakashatsi ku buvuzi bw’ubuvuzi, Minisiteri y’ubuzima y’Uburusiya, Saint-Petersburg, 197341, Uburusiya Tel +7 981 95264800 4997069 Email [imeri irinzwe] Amavu n'amavuko: Uburyo butanga ikizere ku kibazo cyuburozi bwa cytostatike ni ugukoresha magnetiki nanoparticles (MNP) mugutanga ibiyobyabwenge.Intego: Gukoresha ibarwa kugirango umenye ibyiza biranga umurima wa magneti ugenzura MNPs muri vivo, no gusuzuma imikorere ya magnetron yohereza MNPs kubyimba imbeba muri vitro no muri vivo.(MNPs-ICG) ikoreshwa.Muri vivo luminescence ubukana bwakorewe mu mbeba yibibyimba, hamwe na magnetiki yumurima kandi ushimishije.Ubu bushakashatsi bwakorewe kuri hydrodynamic scafold yakozwe n’ikigo cy’ubuvuzi bw’ubushakashatsi bw’ikigo cy’ubushakashatsi cy’ubuvuzi cya Leta cya Almazov cya Minisiteri y’ubuzima y’Uburusiya.Igisubizo: Gukoresha magnesi ya neodymium byateje imbere guhitamo MNP.Umunota umwe nyuma yubuyobozi bwa MNPs-ICG kumbeba zifite ibibyimba, MNPs-ICG yegeranya cyane mwumwijima.Mugihe udahari kandi uhari wa magneti, ibi byerekana inzira ya metabolike.Nubwo kwiyongera kwa fluorescence mubyimba byagaragaye imbere yumurima wa magneti, ubukana bwa fluorescence mumwijima winyamaswa ntabwo bwahindutse mugihe.Umwanzuro: Ubu bwoko bwa MNP, bufatanije nimbaraga zabazwe za magnetique, zishobora kuba intandaro yiterambere ryogukwirakwiza magnetiki kugemura imiti ya cytostatike mumyanya yibibyimba.Ijambo ryibanze: isesengura rya fluorescence, indocyanine, oxyde oxyde nanoparticles, gutanga magnetron ya cytostatics, kwibasira ikibyimba
Indwara yibibyimba nimwe mumpamvu nyamukuru zitera urupfu kwisi yose.Muri icyo gihe, imbaraga zo kongera indwara nimpfu zindwara yibibyimba ziracyahari.1 Chimiotherapie ikoreshwa muri iki gihe iracyari imwe mu miti nyamukuru ivura ibibyimba bitandukanye.Muri icyo gihe, guteza imbere uburyo bwo kugabanya uburozi bwa sisitemu ya cytostatike buracyafite akamaro.Uburyo butanga icyizere cyo gukemura ikibazo cy’uburozi ni ugukoresha abatwara nano-nini kugirango bagere ku buryo bwo gutanga imiti, ishobora gutanga ikwirakwizwa ry’imiti mu ngingo z’ibibyimba bitarinze kwiyongera kwinshi mu ngingo n’umubiri.kwibanda.2 Ubu buryo butuma bishoboka kunoza imikorere no kwibasira imiti ya chimiotherapeutic kumitsi yibibyimba, mugihe bigabanya uburozi bwa sisitemu.
Muri nanoparticles zitandukanye zifatwa mugutanga ibikoresho bya cytostatike, magnetiki nanoparticles (MNPs) zirashimishije cyane kubera imiterere yihariye ya chimique, ibinyabuzima, na magnetique, byemeza ko bihindagurika.Kubwibyo, magnetiki nanoparticles irashobora gukoreshwa nka sisitemu yo gushyushya kuvura ibibyimba hamwe na hyperthermia (hyperthermia).Birashobora kandi gukoreshwa nkibikoresho byo gusuzuma (magnetic resonance diagnosisse).3-5 Ukoresheje ibyo biranga, ufatanije nibishoboka byo kwirundanya kwa MNP mukarere runaka, binyuze mumikoreshereze ya magnetiki yo hanze, gutanga imiti igenewe imiti ifungura uburyo bwo gukora sisitemu ya magnetron ikora cyane kugirango yibasire cytostatike kurubuga rwibibyimba. Ibyiringiro.Sisitemu nkiyi yaba irimo MNP hamwe na magnetique imirima kugirango igenzure ingendo zabo mumubiri.Muri iki gihe, imirima ya magnetiki yo hanze hamwe nogushiraho magnetiki byashyizwe mumubiri urimo ikibyimba birashobora gukoreshwa nkisoko yumurima wa magneti.Uburyo bwa mbere bufite inenge zikomeye, harimo no gukoresha ibikoresho kabuhariwe mu gukoresha magnetiki yibasira ibiyobyabwenge no gukenera guhugura abakozi kubaga.Byongeye kandi, ubu buryo bugarukira ku giciro kinini kandi burakwiriye gusa kubyimba "superficial" hafi yubuso bwumubiri.Ubundi buryo bwo gukoresha magnetique yongeramo uburyo bwo gukoresha ubu buryo bwikoranabuhanga, byorohereza ikoreshwa ryibibyimba biri mubice bitandukanye byumubiri.Byombi bya magneti na magnesi byinjijwe muri stent intinaluminal birashobora gukoreshwa nkibishishwa byangiza ibibyimba mu ngingo zidafite ishingiro kugirango byemeze neza.Nyamara, dukurikije ubushakashatsi bwacu bwite butaratangazwa, ntabwo ari magnetique ihagije kugirango MNP igumane mumaraso.
Imikorere yo gutanga imiti ya magnetron iterwa nibintu byinshi: ibiranga ubwikorezi bwa rukuruzi ubwayo, nibiranga inkomoko yumurima wa magneti (harimo ibipimo bya geometrike ya magnesi zihoraho n'imbaraga z'umurima wa rukuruzi zitanga).Iterambere ryimikorere ya magnetiki iyobora ingirabuzimafatizo ikwirakwiza bigomba kuba bikubiyemo iterambere ryogutwara ibiyobyabwenge bya magnetiki nanoscale, gusuzuma umutekano wabo, no gukora protocole yerekana amashusho yemerera gukurikirana imigendekere yabo mumubiri.
Muri ubu bushakashatsi, twabaze imibare iboneye ya magnetiki yumurima kugirango tugenzure ibiyobyabwenge bya magnetiki nano-nini itwara umubiri.Ikibazo cyo kugumana MNP binyuze mu rukuta rw'imitsi y'amaraso hifashishijwe imbaraga za magneti zikoreshwa hamwe nibi bimenyetso byo kubara byanakozweho ubushakashatsi mu miyoboro y'amaraso yitaruye.Mubyongeyeho, twashushanyije conjugate ya MNPs na fluorescent agent hanyuma dushiraho protocole yo kubonwa kwabo muri vivo.Mubihe bya vivo, mumbeba yerekana ikibyimba, uburyo bwo kwegeranya MNPs mumyanya yibibyimba iyo bikozwe muburyo bwa sisitemu ya magneti.
Mu bushakashatsi bwa vitro, twakoresheje MNP, naho mu bushakashatsi bwa vivo, twakoresheje MNP yometse kuri acide lactique polyester (aside polylactique, PLA) irimo agent ya fluorescent (indolecyanine; ICG).MNP-ICG yashyizwe mu rubanza, koresha (MNP-PLA-EDA-ICG).
Synthesis hamwe numubiri nubumara bya MNP byasobanuwe muburyo burambuye ahandi.7,8
Mu rwego rwo guhuza MNPs-ICG, hakozwe bwa mbere conjugate ya PLA-ICG.Ifu y'amoko ivanze ya PLA-D na PLA-L ifite uburemere bwa molekile 60 kDa.
Kubera ko PLA na ICG byombi ari acide, kugirango dushobore guhuza conjugate ya PLA-ICG, ubanza gukenera guhuza icyogajuru cyarangije amino kuri PLA, gifasha chemisorb ya ICG kuri space.Icyogajuru cyashizwemo hakoreshejwe diamine ya Ethylene (EDA), uburyo bwa karbodiimide hamwe na karubodiimide ikemura amazi, 1-Ethyl-3- (3-dimethylaminopropyl) karbodiimide (EDAC).Umwanya wa PLA-EDA uhujwe kuburyo bukurikira.Ongeramo inshuro 20 zirenze EDA hamwe ninshuro 20 zirenga EDAC kuri 2 mL ya 0.1 g / mL PLA chloroform.Synthesis yakorewe mumashanyarazi ya mL 15 ya polipropilene kuri shaker ku muvuduko wa 300 min-1 mumasaha 2.Gahunda ya synthesis irerekanwa mumashusho 1. Subiramo synthesis hamwe ninshuro 200 zirenga reagent kugirango uhindure gahunda ya synthesis.
Iyo synthesis irangiye, igisubizo cyashyizwemo umuvuduko wa 3000 min-1 muminota 5 kugirango ukureho inkomoko ya polyethylene irenze.Hanyuma, mL 2 yumuti wa 0.5 mg / mL ICG muri dimethyl sulfoxide (DMSO) yongewe kumuti wa 2 mL.Umukangurambaga ashyizwe ku muvuduko ukabije wa 300 min-1 mu masaha 2.Igishushanyo mbonera cya conjugate yabonetse cyerekanwe mubishusho 2.
Muri 200 mg MNP, twongeyeho 4 mL PLA-EDA-ICG conjugate.Koresha shitingi ya LS-220 (LOIP, Uburusiya) kugirango uhagarike guhagarika iminota 30 kuri frequence ya 300 min-1.Hanyuma, yogejwe hamwe na isopropanol inshuro eshatu hanyuma itandukana na magneti.Koresha UZD-2 Ultrasonic Disperser (FSUE NII TVCH, Uburusiya) kugirango wongere IPA kumuhagarikwa muminota 5-10 mugihe gikomeje ultrasonic.Nyuma yo gukaraba IPA ya gatatu, imvura yogejwe namazi yatoboye hanyuma ihagarikwa muri saline physiologique yibiro bya mg / mL 2.
Ibikoresho bya ZetaSizer Ultra (Malvern Instruments, UK) byakoreshejwe mu kwiga ingano yo gukwirakwiza MNP yabonetse mu gisubizo cy’amazi.Ikwirakwizwa rya elegitoronike ya microscope (TEM) hamwe na JEM-1400 STEM yoherezwa mu kirere (JEOL, Ubuyapani) yakoreshejwe mu kwiga imiterere n'ubunini bwa MNP.
Muri ubu bushakashatsi, dukoresha magnesi zihoraho (icyiciro cya N35; hamwe na nikel ikingira ikingira) hamwe nubunini bukurikira (uburebure bwa axis ndende diameter diameter): 0.5 × 2 mm, 2 × 2 mm, 3 × 2 mm na 5 × 2 mm.
Ubushakashatsi bwakozwe na vitro ku bwikorezi bwa MNP muri sisitemu y’icyitegererezo bwakorewe kuri hydrodynamic scafold yakozwe n’ikigo cy’ubuvuzi bw’ubushakashatsi bw’ikigo cy’ubushakashatsi cy’ubuvuzi cya Leta cya Almazov cya Minisiteri y’ubuzima y’Uburusiya.Ingano yamazi azenguruka (amazi yatoboye cyangwa igisubizo cya Krebs-Henseleit) ni 225 mL.Imashini ya magnetiki ya silindrike ikoreshwa nka magnesi zihoraho.Shira rukuruzi kuri mm 1,5 uvuye kurukuta rwimbere rwikirahure rwagati, hamwe numutwe wacyo werekeza ku cyerekezo cyumuyoboro (vertical).Igipimo cyamazi mumuzinga ufunze ni 60 L / h (bihuye numuvuduko wumurongo wa 0.225 m / s).Krebs-Henseleit igisubizo gikoreshwa nkamazi azenguruka kuko nikigereranyo cya plasma.Coefficient ya viscosity ya plasma ni 1.1-1.3 mPa ∙ s.Ingano ya MNP yamamajwe mu murima wa magneti igenwa na spekitifotometometrie iva mu cyuma cya fer mu mazi azenguruka nyuma yubushakashatsi.
Byongeye kandi, ubushakashatsi bwubushakashatsi bwakorewe kumeza yubukanishi bwamazi meza kugirango hamenyekane neza imiyoboro yimitsi.Ibice byingenzi bigize inkunga ya hydrodynamic bigaragara mu gishushanyo cya 3. Ibice byingenzi bigize stent ya hydrodynamic ni umugozi ufunze ugereranya ibice byambukiranya sisitemu yimitsi yimitsi hamwe nigikoresho cyo kubikamo.Kugenda kwamazi yicyitegererezo kumurongo wa module yamaraso itangwa na pompe ya peristaltike.Mugihe cyigeragezwa, komeza umwuka hamwe nubushyuhe bukenewe, kandi ukurikirane ibipimo bya sisitemu (ubushyuhe, umuvuduko, umuvuduko wamazi, nagaciro ka pH).
Igishushanyo cya 3 Hagarika igishushanyo mbonera cyakoreshejwe mukwiga uburyo bwurukuta rwimitsi ya karoti.Ikigega cyo kubika 1, pompe 2-peristaltike, uburyo-3 bwo kwinjiza ihagarikwa ririmo MNP mu cyerekezo, metero 4 zitemba, icyuma gikoresha ingufu 5 mu cyerekezo, guhinduranya ubushyuhe 6, icyumba 7 gifite kontineri, 8-isoko yumurima wa magneti, 9-ballon hamwe na hydrocarbone.
Icyumba kirimo kontineri kigizwe na kontineri eshatu: ikintu kinini cyo hanze n’ibikoresho bibiri bito, unyuramo amaboko yumuzunguruko wo hagati.Urumogi rwinjijwe mu kintu gito, icyombo gitsindagiye ku kintu gito, kandi isonga ry'urumogi rihambiriwe cyane n'umugozi muto.Umwanya uri hagati yikintu kinini nigikoresho gito cyuzuyemo amazi yatoboye, kandi ubushyuhe buguma buhoraho kubera guhuza nubushyuhe.Umwanya uri muri kontineri nto wuzuyemo igisubizo cya Krebs-Henseleit kugirango ukomeze kubaho neza kwingirangingo zamaraso.Ikigega nacyo cyuzuyemo igisubizo cya Krebs-Henseleit.Sisitemu yo gutanga gaze (karubone) ikoreshwa mu guhumeka igisubizo mu kintu gito kiri mu kigega cyo kubikamo no mu cyumba kirimo kontineri (Ishusho 4).
Igishushanyo 4 Icyumba gishyizwemo kontineri.1-Cannula yo kugabanya imiyoboro yamaraso, 2-Icyumba cyo hanze, 3-Icyumba gito.Umwambi werekana icyerekezo cyamazi yicyitegererezo.
Kugirango umenye igipimo cyerekana igipimo cyurukuta rwubwato, imiyoboro yimbeba karotide yakoreshejwe.
Kwinjiza ihagarikwa rya MNP (0.5mL) muri sisitemu bifite ibimenyetso bikurikira: ubwinshi bwimbere bwikigega hamwe numuyoboro uhuza umuzenguruko ni 20mL, naho imbere muri buri cyumba ni 120mL.Inkomoko ya magnetiki yo hanze ni magneti ahoraho afite ubunini busanzwe bwa 2 × 3 mm.Yashyizwe hejuru yimwe mubyumba bito, cm 1 uvuye kuri kontineri, numutwe umwe ureba urukuta rwa kontineri.Ubushuhe bubikwa kuri 37 ° C.Imbaraga za pompe ya roller yashyizwe kuri 50%, bihuye n'umuvuduko wa cm 17 / s.Nkigenzura, ingero zafatiwe muri selire idafite magnesi zihoraho.
Nyuma yisaha imwe nyuma yubuyobozi bwa MNP bwatanzwe, icyitegererezo cyamazi cyakuwe mubyumba.Ubwinshi bwibice byapimwe na spekitifotometero ikoresheje Unico 2802S UV-Vis spectrophotometer (United Products & Instruments, USA).Urebye uburyo bwo kwinjiza ibintu bya MNP ihagarikwa, gupima byakorewe kuri 450 nm.
Dukurikije amabwiriza ya Rus-LASA-FELASA, inyamaswa zose zororerwa kandi zikororerwa mu bigo byihariye bidafite indwara.Ubu bushakashatsi bwubahirije amabwiriza yose agenga imyitwarire y’ubushakashatsi bw’inyamaswa n’ubushakashatsi, kandi yemerewe imyitwarire n’ikigo cy’ubushakashatsi cy’ubuvuzi cya Almazov (IACUC).Inyamaswa zanyweye amazi ad libitum kandi zigaburirwa buri gihe.
Ubushakashatsi bwakorewe ku mbeba 10 zatewe n’ibyumweru 12 by’imbeba zidafite ubudahangarwa bw’umugabo (NOD.Cg-Prkdcscid Il2rgtm1Wjl / Szj, Laboratoire ya Jackson, muri Amerika) 10, ipima 22 g ± 10%.Kubera ko ubudahangarwa bwimbeba za immunodeficiency zahagaritswe, imbeba zo gukingira indwara zuyu murongo zemerera kwimura ingirabuzimafatizo hamwe nuduce twanze kwangwa.Abanywanyi bava mumagage atandukanye bashizwe mumurwi wubushakashatsi, kandi barororerwa hamwe cyangwa bahujwe nuburiri bwandi matsinda kugirango barebe ko mikorobe isanzwe ihura.
Umurongo wa kanseri ya kanseri ya HeLa ukoreshwa mugushiraho icyitegererezo cya xenograft.Ingirabuzimafatizo zarezwe muri DMEM zirimo glutamine (PanEco, Uburusiya), hiyongeraho 10% ya serumu y'inda y'inda (Hyclone, Amerika), 100 CFU / mL penisiline, na 100 μg / mL streptomycine.Umurongo w'akagari watanzwe neza na Laboratoire ya Gene Expression Regulation Institute of Institute Research Cell of the Academy of Science of Russian.Mbere yo gutera inshinge, selile HeLa yakuwe muri plastiki yumuco hamwe na trypsin 1: 1: Umuti wa Versene (Biolot, Uburusiya).Nyuma yo gukaraba, selile zahagaritswe muburyo bwuzuye kugeza kuri selile 5 × 106 kuri 200 μL, hanyuma zivangwa na matrix yo munsi (LDEV-KUBUNTU, MATRIGEL® CORNING®) (1: 1, kurubura).Guhagarika ingirabuzimafatizo byateguwe byatewe mu ruhu rwibibero byimbeba.Koresha ibyuma bya elegitoronike kugirango ukurikirane imikurire yikibyimba buri minsi 3.
Iyo ikibyimba kigeze kuri mm3 500, magneti ahoraho yatewe mumitsi yinyamanswa yubushakashatsi hafi yikibyimba.Mu itsinda ryubushakashatsi (MNPs-ICG + tumor-M), 0.1 mL yo guhagarika MNP yatewe kandi ihura numurima wa rukuruzi.Inyamaswa zose zitavuwe zakoreshejwe nkigenzura (inyuma).Byongeye kandi, hakoreshejwe inyamaswa zatewe na 0.1 mL ya MNP ariko zidatewe na magnesi (MNPs-ICG + tumor-BM).
Amashusho ya fluorescence yo muri vivo no muri sample ya vitro yakorewe kuri bioimager ya IVIS Lumina LT III (PerkinElmer Inc., USA).Kubijyanye na vitro visualisation, ingano ya 1 mL ya synthique PLA-EDA-ICG na MNP-PLA-EDA-ICG conjugate yongewe kumariba yisahani.Urebye ibiranga fluorescence iranga irangi rya ICG, hatoranijwe akayunguruzo keza gakoreshwa mukumenya ubukana bwurumuri rwicyitegererezo cyatoranijwe: uburebure bwumurambararo mwinshi ni 745 nm, naho uburebure bwacyo ni 815 nm.Porogaramu Nzima 4.5.5 software (PerkinElmer Inc.) yakoreshejwe mu gupima ingano ubukana bwa fluorescence y'amariba arimo conjugate.
Ubukomezi bwa fluorescence hamwe no kwegeranya kwa MNP-PLA-EDA-ICG conjugate byapimwe mu mbeba zerekana imiterere ya vivo ikibyimba, hatabayeho kubaho no gukoresha umurima wa rukuruzi ahantu ushimishijwe.Imbeba zatewe aneste hamwe na isoflurane, hanyuma 0.1 mL ya MNP-PLA-EDA-ICG conjugate yatewe mumitsi.Imbeba zitavuwe zakoreshejwe nkigenzura ribi kugirango ubone florescente.Nyuma yo gutanga conjugate imitsi, shyira inyamanswa kurwego rwo gushyushya (37 ° C) mucyumba cya IVIS Lumina LT ya III fluorescence imager (PerkinElmer Inc.) mugihe ukomeza guhumeka hamwe na 2% isoflurane anaesthetisation.Koresha ICG yubatswe muyunguruzi (745–815 nm) kugirango umenye ibimenyetso umunota 1 niminota 15 nyuma yo gutangiza MNP.
Kugira ngo hamenyekane ikwirakwizwa rya conjugate mu kibyimba, agace ka peritoneyale y’inyamaswa kari gatwikiriye impapuro, bituma bishoboka gukuraho florescence yaka cyane ijyanye no kwegeranya uduce duto mu mwijima.Nyuma yo kwiga ikwirakwizwa ry’ibinyabuzima rya MNP-PLA-EDA-ICG, inyamaswa zahawe abantu mu buryo bukabije n’ikirenga cya anesthesia ya isoflurane kugira ngo itandukane nyuma y’ibibyimba no gusuzuma umubare w’imirasire ya fluorescence.Koresha Ishusho Nzima 4.5.5 software (PerkinElmer Inc.) kugirango ukoreshe intoki isesengura ryibimenyetso bivuye mukarere katoranijwe.Ibipimo bitatu byafashwe kuri buri nyamaswa (n = 9).
Muri ubu bushakashatsi, ntitwigeze tugereranya uburyo bwo gupakira neza ICG kuri MNPs-ICG.Mubyongeyeho, ntabwo twagereranije imikorere yo kugumana nanoparticles iyobowe na magnesi zihoraho zuburyo butandukanye.Twongeyeho, ntitwasuzumye ingaruka ndende z'umurima wa magneti ku kugumana kwa nanoparticles mu ngingo z'ibibyimba.
Nanoparticles yiganje, hamwe nimpuzandengo ya 195.4 nm.Mubyongeyeho, ihagarikwa ryarimo agglomerates ifite impuzandengo ya 1176.0 nm (Ishusho 5A).Ibikurikira, igice cyayungurujwe binyuze muri centrifugal filter.Ubushobozi bwa zeta bwibice ni -15,69 mV (Ishusho 5B).
Igicapo 5 Ibintu bifatika byo guhagarikwa: (A) ingano yubunini;(B) gukwirakwiza ibice kuri zeta ubushobozi;(C) Ifoto ya TEM ya nanoparticles.
Ingano yingirakamaro ni 200 nm (Igicapo 5C), igizwe na MNP imwe ifite ubunini bwa nm 20, hamwe na PLA-EDA-ICG ihujwe nigishishwa kama gifite ubucucike buke bwa electron.Ihinduka rya agglomerates mubisubizo byamazi birashobora gusobanurwa na modulus iri munsi yingufu za electromotive imbaraga za nanoparticles.
Kuri magnesi zihoraho, mugihe magnetisation yibanda mubunini bwa V, imvugo yibanze igabanijwemo ibice bibiri, aribyo ingano n'ubuso:
Mugihe cyicyitegererezo hamwe na magnetisiyasi ihoraho, ubucucike buriho ni zeru.Hanyuma, imvugo ya magnetic induction vector izafata uburyo bukurikira:
Koresha gahunda ya MATLAB (MathWorks, Inc., USA) kugirango ubare umubare, ETU “LETI” uruhushya rwamasomo nimero 40502181.
Nkuko bigaragara ku gishushanyo cya 7 Igicapo 8 Igicapo 9 Igicapo-10, umurima ukomeye wa magneti ukorwa na rukuruzi yerekanwe mu buryo bwa axe kuva kumpera ya silinderi.Iradiyo ikora neza ihwanye na geometrie ya magneti.Muri magnetiki ya silindrike ifite silinderi ifite uburebure burenze diameter, umurima ukomeye wa magnetique ugaragara mubyerekezo bya axial-radial (kubice bihuye);Kubwibyo, couple ya silinderi ifite igipimo kinini kinini (diameter n'uburebure) MNP adsorption niyo nziza cyane.
Igishushanyo 7 Ibigize ubukana bwa magnetique induction Bz kuruhande rwa Oz axis ya rukuruzi;ubunini busanzwe bwa magneti: umurongo wumukara 0.5 × 2mm, umurongo wubururu 2 × 2mm, umurongo wicyatsi 3 × 2mm, umurongo utukura 5 × 2mm.
Igicapo 8 Igice cya magnetiki induction Br ni perpendicular kuri magnet axis Oz;ubunini busanzwe bwa magneti: umurongo wumukara 0.5 × 2mm, umurongo wubururu 2 × 2mm, umurongo wicyatsi 3 × 2mm, umurongo utukura 5 × 2mm.
Igicapo 9 Imbaraga za magnetiki induction ubukana bwa Bz intera r kuva kumurongo wanyuma wa magneti (z = 0);ubunini busanzwe bwa magneti: umurongo wumukara 0.5 × 2mm, umurongo wubururu 2 × 2mm, umurongo wicyatsi 3 × 2mm, umurongo utukura 5 × 2mm.
Igishushanyo cya 10 Magnetic induction yibice byerekezo ya radiyo;ingano ya magneti isanzwe: umurongo wumukara 0.5 × 2mm, umurongo wubururu 2 × 2mm, umurongo wicyatsi 3 × 2mm, umurongo utukura 5 × 2mm.
Moderi idasanzwe ya hydrodinamike irashobora gukoreshwa mukwiga uburyo bwo gutanga MNP kumubiri wibibyimba, kwibanda kuri nanoparticles mukarere kateganijwe, no kumenya imyitwarire ya nanoparticles mubihe bya hydrodynamic muri sisitemu yo gutembera.Imashini zihoraho zirashobora gukoreshwa nkumurima wa magneti wo hanze.Niba twirengagije imikoranire ya magnetostatike hagati ya nanoparticles kandi ntituzirikane urugero rwamazi ya magnetique, birahagije kugereranya imikoranire hagati ya magneti na nanoparticle imwe hamwe na dipole-dipole igereranijwe.
Aho m nigihe cya magnetiki ya magnet, r ni radiyo ya vector ya point ya nanoparticle iherereye, na k nikintu cya sisitemu.Kugereranya dipole, umurima wa magneti ufite ibisa bisa (Ishusho 11).
Mu murima umwe rukuruzi, nanoparticles izunguruka gusa kumurongo wimbaraga.Mu murima wa magneti udahuje, imbaraga zikora kuri yo:
Nihe inkomoko yicyerekezo cyatanzwe l.Mubyongeyeho, imbaraga zikurura nanoparticles mubice bitaringaniye byumurima, ni ukuvuga kugabanuka nubucucike bwimirongo yingufu byiyongera.
Kubwibyo, ni byiza gukoresha magnet akomeye (cyangwa urunigi rukuruzi) hamwe na anisotropy igaragara ya axial mugace aho ibice biherereye.
Imbonerahamwe 1 irerekana ubushobozi bwa rukuruzi imwe nkisoko ihagije ya magneti yo gufata no kugumana MNP muburiri bwimitsi yumurima wabisabye.